The World Library of Toxicology Reagents (WLTR), obtainable from the National Institute of Environmental Health Sciences (NIEHS), comprises a diverse range of toxicological reagents. These include hepatocytes, cytochrome P450 enzymes, S9 fractions, microsomes, and other essential elements extensively employed in toxicology research. The reagents offered by WLTR encompass various species such as human, rat, and beagle.
A selection of the available World Library of Toxicology Reagents includes:
- Hepatocytes: Human, rat, beagle
- Cytochrome P450 enzymes: CYP1A2, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP3A4
- S9 fractions: Human, rat, beagle
- Microsomes: Human, rat, beagle
- Other reagents: Luciferase, NADPH, cytochrome b5, UDP-glucuronosyltransferase
These WLTR reagents find applications in diverse areas of toxicology research, including:
- Studies on drug metabolism and toxicity
- Research on environmental toxicology
- Mechanistic toxicology studies
- Risk assessment endeavors
These reagents are accessible for purchase on the NIEHS website, which also provides an array of resources to aid researchers in utilizing the WLTR reagents effectively. These resources encompass protocols, technical support, and training.
Hepatocytes, responsible for processing drugs and other chemicals in the liver, are extensively utilized in toxicology research to examine the metabolism and toxicity of various substances.
Cytochrome P450 enzymes, constituting a vital enzyme family involved in drug and chemical metabolism, are instrumental in scrutinizing the role of these enzymes in the processing and toxicity of different compounds.
S9 fractions, representing subcellular portions of liver cells comprising cytochrome P450 enzymes and other metabolically relevant enzymes, are utilized in vitro for investigating the metabolism and toxicity of substances.
Similarly, microsomes, another subcellular fraction of liver cells containing essential enzymes, serve as a key tool in understanding the metabolism and toxicity of various substances in vitro.
These insights merely scratch the surface of the extensive repertoire of the World Library of Toxicology Reagents available for acquisition. For further details, kindly refer to the NIEHS website.